Electrophysiology and data analysis
نویسندگان
چکیده
Synaptic currents were recorded in HL-3 saline from muscle 6 in segment A2 using a twoelectrode voltage clamp. For EJC recordings, stimuli consisted of 0.2 ms pulses delivered at 1 Hz from an isolated pulse stimulator (AM systems 2100), gated by pClamp software (Axon Instruments). Intracellular glass microelectrodes were filled with 3 M KCl (voltage monitor electrode, resistance 7–10 MQ) or saturated potassium citrate and 3 M KCl (current injection electrode, resistance 18–25 MQ). Data were acquired from muscles clamped at 270 mV, using an Axoclamp 2B amplifier (Axon Instruments), and recorded with pClamp software. Data were filtered at 1 kHz before analysis. EJC amplitude was determined from 22–25 contiguous stimuli, and mEJC parameters were measured from one to two 41-s records, using the minianalysis software (Jaejin Software). Differences between means were compared using either t-tests (paired comparisons) or analysis of variance (ANOVA; multiple comparisons) in Microsoft Excel or SigmaPlot.
منابع مشابه
A Comparison of Two Electrophysiology Centers in Different Social And Economic Regions Of Turkey During First Quarter of the Year
Introduction: More and more patients have been undergoing electrophysiological study (EPS) as the number of rhythmologists have increased. Due to the increased interest in the study, today EPS applications are made even in second step public hospitals or private hospitals. Our aim is to compare two electrophysiology labs, that are in different regions with social and economic development, in te...
متن کاملNovel frameshift mutation in the KCNQ1 gene responsible for Jervell and Lange-Nielsen syndrome
Objective(s): Jervell and Lange–Nielsen syndrome is an autosomal recessive disorder caused by mutations in KCNQ1 or KCNE1 genes. The disease is characterized by sensorineural hearing loss and long QT syndrome. Methods: Here we present a 3.5-year-old female patient, an offspring of consanguineous marriage, who had a history of recurrent syncope and congenital sensorineural deafness. The patient ...
متن کاملEvaluation of Patient's Dose and Estimate of Cancer Risk in Electrophysiology Studies and Ablation in Cath Lab Center of Afshar Hospital, Yazd, Iran
Introduction: Today electrophysiology studies and ablation have been developed due to increasing arrhythmias disorder of heart. In these diagnostic – treatments methods, the use of fluoroscopy can be causes patient radiation dose, therefore evaluation of patient's absorbed dose is necessary to protection of the radiation. The aim of this study was to evaluate the absorbed dose in patients under...
متن کاملEffect of Administration of Lidocaine at Body Temperature on Anesthesia Success in Rodent Model: A Behavior and Electrophysiology Study
Introduction: Success in anesthesia administration relieves the perception of pain during surgery. Lidocaine is the most commonly used local anesthetic agent in clinical medicine. Moreover, anesthetic agents' temperature changes can influence cell membrane permeability. Here, the effectiveness of different temperatures of lidocaine (Lid.) on anesthesia success rate in rats has been investigated...
متن کاملTherapeutic effects of ellagic acid on memory, hippocampus electrophysiology deficits, and elevated TNF-α level in brain due to experimental traumatic brain injury
Objective(s): Cognitive defects such as learning and memory impairment are amongst the most repetitious sequelae after sever and moderate traumatic brain injury (TBI). It was suggested that ellagic acid (EA), an innate phenol product, display neuroprotective properties against oxidative and inflammatory damages after brain injury. The object of the current study was therapeutic properties of EA...
متن کاملIdentification of a Novel KCNQ1 Frameshift Mutation and Review of the Literature among Iranian Long QT Families
Background: Long QT syndrome (LQTS) is characterized by the prolongation of QT interval, which results in syncope and sudden cardiac death in young people. KCNQ1 is the most common gene responsible for this syndrome. Methods: Molecular investigation was performed by DNA Sanger sequencing in Iranian families with a history of syncope. In silico examinations were performed for predicting the path...
متن کامل